A growing body of evidence supports the argument that bone marrow-derived mesenchymal stem cells (MSCs) can differentiate\ninto cardiomyocyte-like cells in an appropriate cellular environment, but the differentiation rate is low. A cocktail method was\ndesigned: we investigated the role of 5-azacytidine (5-aza), salvianolic acid B (SalB), and cardiomyocyte lysis medium (CLM) in\ninducing MSCs to acquire the phenotypical characteristics of cardiomyocytes. The fourth-passage MSCs were treated with 5-aza,\nSalB, CLM, 5-aza+salB, 5-aza+CLM, SalB+CLM, and 5-aza+SalB+CLM for 2 weeks. Immunofluorescence results showed that cTnT\nexpression in the 5-aza+salB+CLM group was stronger than other groups. Real-time qPCR andWestern blotting analyses showed\nthat cTnT, alpha-cardiac actin, mef-2c, Cx43, and GSK-3beta expression increased while beta-catenin expression decreased. The\nsalB+5-aza+CLM group had the most evident effects. SalB combined with 5-aza and CLM improved cardiomyocyte differentiation\nfrom MSCs. In the MSCs differentiation process, theWnt/beta-catenin signaling pathway had been inhibited.
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